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Genome and Biomolecular Engineering for Drug Discovery

Prof. NOMURA Wataru

¡¾Research Keyword¡¿
Bioluminescence Resonance Energy Transfer (BRET), Chemical Biology, Chemical Screening, DNA methylation, DNA recombination, Genome Editing, Genome Engineering, Molecular Evolution, Protein Engineering, Signal Transduction, Transcription Regulation

¡¾Recent highlights¡¿
Nomura laboratory has started as of April 1, 2019. Genome editing is a novel technology with possibility of application in many fields including medicine, drug discovery, and animal/plant breeding. We tackle to develop a novel technology in the field to obtain further  knowledge in future. Gene regulation is one of the applicable topics derived from development of genome editing technology. Recently, we have developed a method to regulation orthogonal genes in a single cell (Nomura W, et al. Biochemistry 2019). We also focus on the method to suppress off-target effects for precise genome editing.

¡¾Major Papers of the Laboratory¡¿
?TALEN-Based Chemically Inducible, Dimerization-Dependent, Sequence-Specific Nucleases, Biochemistry, 59(2), 197-204, 20200121
?A cell cycle-dependent CRISPR-Cas9 activation system based on an anti-CRISPR protein shows improved genome editing accuracy, Communications Biology, 3(1), 20201201
?Molecular Switch Engineering for Precise Genome Editing, BIOCONJUGATE CHEMISTRY, 32(4), 639-648, 20210421

·¡»å³Ü³¦²¹³Ù¾±´Ç²Ô¡¿
Classes for undergraduate students: Biochemistry III/Biochemistry IV/Physiological Chemistry/Experiments of Biological Chemistry/Special Laboratory Works
Biochemistry III/IV: the structure, metabolism and physiological role of carbohydrates and lipids
Experiments of Biological Chemistry: learn and practice about enzyme kinetics
Special Laboratory Works: learn and practice about basic techniques/skils of biochemistry, cell biology, and molecular biology. With those practices, students will perform research on their own projects (mainly genome editing and gene regulation) based on topics in the lab.

¡¾¸é±ð²õ±ð²¹°ù³¦³ó¡¿
1.   Genome editing using DNA recombinases
2.   Suppression of off-target effects for precise genome editing
3.   Epigenome editing using DNA methylases
4.   Gene regulation by artificial transcription factors
5.   Chemical biology by protein engineering and genome editing
6.   Visualization of in situ protein dynamics using fluorescent imaging
7.   BRET-based compound screening for drug discovery
8.   Roles of phosphoinositides in signal transducion system
9.   Roles of phosphoinositides in intracellular vesicular trafficking
10. Research on tumor metabolism


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School of Pharmaceutical Sciences

1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan 734-8553
TEL: +81-82-257-1705